Transcriptome analysis of the immunomodulation by Arctium lappa L. polysaccharides in the Chinese mitten crab Eriocheir sinensis against Aeromonas hydrophila

Arctium lappa L. polysaccharide (designated ALP) has been used as an immunomodulator in animals. Immunomodulation by ALP the Chinese mitten crab Eriocheir sinensis was detected after Aeromonas hydrophila treatment (1.0 × 107 colony-forming units) this study. significantly inhibited decrease total hemocyte count (THC) (P < 0.05). Twenty transcriptome libraries were constructed from hemocytes of crabs, and total, 600,827,724 reads 794,113 transcripts obtained clustered into 337,358 unigenes, which annotated CDD, KOG, NR, NT, PFAM, TrEMBL, GO KEGG databases. The differentially expressed gene (DEG) analysis indicated that 97,800 genes upregulated 103,745 downregulated, among 2616 932 immune-related DEGs enriched according to annotations at 12 h, respectively (Q value<0.05). EsDorsal, EsPI3K, EsLectin EsLGBP mRNA upregulated, STAT downregulated A. injection. increase EsDorsal EsPI3K expression promoted expression. alkaline phosphatase (AP) glutamyl transpeptidase (GT) activities supernatant hemolymph increased treatment, immunofluorescence assay could inhibit upregulation TNF-α IL-6 E. challenged h. Therefore, it concluded played important role effective immunomodulation crabs (E. sinensis) during infection.